NPC
在死亡细胞的世界里存在形形色色的NPC。有的人会在你的前进道路上一直陪伴着你，有的人与你，却仅仅是萍水相逢。。。
| NPC名 | 描述 | 位置 | 外观 |
|---|---|---|---|
| 教程骑士 | 玩家初次进入游戏时，女骑士会出现并引导玩家。每当枭首者出逃时，她都会说一些高深莫测的谏言。也许正是因为话语给她惹来了杀身之祸————她在游戏进展不久后就被发现死在了有罪者大道的入口，凶手不知所踪。每当你经过时，你都会看见一具腐臭的尸骨。
|
被囚者牢房 |  |
| 收藏家 | 收藏家会把你在游戏过程中收集的图纸保管起来。当你攒够细胞后，就可以找他永久解锁图纸中的物品。他是推进整个游戏不可或缺的角色（各种意义上的）。
收藏家会在5 BOSS细胞难度下消失不见，让你无处花费细胞。 |
在两个关卡之间的过渡房间(5BC除外) |  |
| Guillain | Lets the player choose a mutation to acquire after every completed biome, with a max ammount of 3 at the same time. Those can be restarted and reselected by a price which increases with each use. | In the transition room located between each two biomes. |  |
| The Blacksmith | The only access the player has to the Legendary Forge, where they can permanently upgrade the quality of the gear found throughout the game. | In the transition room after each boss biome (after the Black Bridge, Insufferable Crypt or the Nest, and after the Clock Room or Guardian's Haven). |  |
| The Tailor | Lets the player change the Beheaded's outfit, choosing between those they have unlocked. | Prisoners' Quarters |  |
| The Scribe | Keeps track of player's game stats. | Prisoners' Quarters |  |
| The Spider | Explains the Darkness mechanic at the entrance of the Forgotten Sepucher. | At the entrance of the Forgotten Sepulcher |  |
| The Ghost | Explains how challenge rifts work, the first time one is entered. | At the entrance of the first Challenge Rift the player enters |  |
| The King | Throne Room |  | |
| Gollum | Toxic Sewers |  |
已移除的NPC
| NPC name | Info | Location | Image |
|---|---|---|---|
| The Fisherman | NPC that was found in the Pier and, when spoken to, would kill the Beheaded to let him start a new run. | Pier |  |